Figure 5.
Induction of TRIB1 gene expression and downregulation of lipogenic genes by BBR in human liver cells. (A) HepG2 cells were seeded in 12-well cell culture plate (5 × 105cell/well) and treated with BBR (40 μM) in duplicate wells for different time intervals as indicated. Total RNA was isolated from treated cells for real-time PCR analysis. The data shown are mean ± S.E.M. of two RNA samples with triplicate measurement per RNA sample. (B) HPHs were treated with BBR (20 μM) or DMSO for 3 h in triplicate wells. TRIB1 mRNA levels were determined by real-time PCR. The data shown are mean ± S.E.M. of three RNA samples with triplicate measurements per RNA sample. *P < 0.05, **P < 0.01, and ***P < 0.001, compared with DMSO control.