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. 2019 Oct 30;9:15693. doi: 10.1038/s41598-019-52065-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OptiJ plugin library workflow for the correction of common OPT artifacts. (a) Typical workflow for the use of the OptiJ plugins. (b) Correction of common OPT artifacts using OptiJ plugins. The top row represents images without correction applied (w/o). The bottom row shows images after correction (w). (i) Uneven illumination in raw tOPT projections resulting from the optics used to collimate the light source, and absorption and scattering from the sample. (ii) Shadow artifact originating from a misalignment of the sample’s axis of rotation. (iii) Jittered sinogram of a marker bead rotated by a low-cost stepper motor.