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. 2019 Oct 24;10:1283. doi: 10.3389/fphar.2019.01283

Figure 5.

Figure 5

8-Formylophiopogonanone B (8-FOB) mitigates paraquat (PQ)-induced apoptosis in L02 cells. (A) Representative images showing the effects of 8-FOB inhibition on PQ-induced apoptosis determined by flow cytometry. The annexin V–fluorescein isothiocyanate (FITC)−/propidium iodide (PI)− population consists of normal healthy cells (Q4); annexin V–FITC+/PI− cells indicate early apoptosis (Q3); annexin V–FITC+/PI+ cells indicate late apoptosis or necrosis (Q2); annexin V–FITC−/PI+ cells are necrotic cells (Q1). (B) Quantification of apoptosis ratios. (C) Detection of caspase-3 activity after 24 h of PQ (500 μM) treatment. Ac-DEVD-CHO: a specific caspase-3 inhibitor. (D) Detection of caspase-3 expression levels after 24 h of PQ (500 μM) treatment. (E) Quantification of cleaved caspase-3 levels relative to those of β-actin. The values are presented as the means ± SEM (n = 6). *p < 0.05, **p < 0.01 vs the control group; # p < 0.05, ## p < 0.01 vs the PQ group.