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. 2019 Oct 30;9:15665. doi: 10.1038/s41598-019-52169-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of intracellular glucose and glutamine levels and proliferation in glucose or glutamine deprived environment of MCF-7 cells. (A) Intracellular glucose level determined by a fluorescence based assay. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. (B) Cell proliferation analysis under control (4500 mg/l glucose) and low glucose (1000 mg/l glucose) concentration. Data are presented as a mean of n = 4 ± SEM. Unpaired t test with Welch’s correction. (C) Absolute glutamine level quantified by a colorimetric assay. Data are presented as a mean of n = 4 ± SEM. (D) Cell proliferation under control glutamine supplementation (1% GlutaMax) and without glutamine supplementation. Data are presented as a mean of n = 3–9 ± SEM. Unpaired t test with Welch’s correction. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.