Table 2.
Genetic diversity within Miscanthus sacchariflorus and M. × giganteus collected in eastern Asia
DAPC group | No. of individuals | SNP diversity (D) | Mean extrapolated allelic richness* | Number of SNPs with minor allele frequency >0.05 | N P | N P unique | Plastid Gini–Simpson index | Plastid Gini–Simpson index excluding Msi haplotypes |
---|---|---|---|---|---|---|---|---|
Yangtze diploids (ssp. lutarioriparius) | 67 | 0.115 | 1.71 | 11 377 | 17 | 5 | 0.69 ± 0.06 | 0.69 ± 0.06 |
N China diploids | 64 | 0.139 | 1.82 | 15 087 | 18 | 3 | 0.85 ± 0.03 | 0.85 ± 0.03 |
Korea/NE China/Russia diploids | 256 | 0.141 | 1.89 | 14 778 | 20 | 9 | 0.85 ± 0.01 | 0.85 ± 0.01 |
N China/Korea/Russia tetraploids | 139 | 0.133 | 1.94 | 14 648 | 19 | 7 | 0.79 ± 0.03 | 0.79 ± 0.03 |
S Japan tetraploids | 120 | 0.139 | 1.89 | 15 509 | 20 | 6 | 0.75 ± 0.04 | 0.74 ± 0.04 |
N Japan tetraploids | 76 | 0.134 | 1.80 | 14 249 | 9 | 2 | 0.46 ± 0.07 | 0.45 ± 0.07 |
Mxg (2x × 2x) | 7 | 0.123 | 1.82 | 12 616 | 5 | 0 | 0.86 ± 0.10 | 0.86 ± 0.10 |
Mxg (4x × 2x) | 30 | 0.187 | 1.86 | 18 897 | 9 | 0 | 0.77 ± 0.06 | 0.53 ± 0.13 |
Groupings are based on discriminant analysis of principal components (DAPC) for 34 605 RAD-seq SNPs (Fig. 2).
Msi, M. sinensis; Mxg, M. × giganteus; D, diversity, as calculated from expected heterozygosity of RAD-seq SNPs (allele frequencies were estimated from read count data using the R package polyfreqs for tetraploid and triploid groups, and directly from genotypes called with UNEAK for diploid groups; standard error of D = 0.001 for each group); NP, number of plastid haplotypes; NP unique, number of plastid haplotypes unique to each group.
*Standard error of 0.001 or 0.002 for each group for allelic richness.