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. 2019 Oct 30;9:15625. doi: 10.1038/s41598-019-52176-8

Figure 2.

Figure 2

MMV675968 inhibits AbDHFR but not hDHFR in an E. coli model. (A) AbDHFR and hDHFR can complement for the loss of EcDHFR. Cultures of E. coli PA414 (DHFR−, TS−) carrying empty plasmids or plasmids expressing EcTS and either AbDHFR or hDHFR were spotted onto LB plate supplemented with 50 µg/ml thymidine or 0.2% arabinose. A representative picture from three independent experiments is shown. (B) MMV675968 inhibits the growth of E. coli model expressing AbDHFR not hDHFR when grown without thymidine supplement. (C) Thymidine supplement rescues growth inhibition of E. coli surrogate expressing EcTS and AbDHFR by MMV675968. E. coli PA414 expressing EcTS and either AbDHFR or hDHFR were incubated with 10 µM of DHFR inhibitors for 18 hours. Growth was monitored using OD600. Percent growth was calculated relative to untreated which was set at 100%. The graph shows mean ± SEM from three independent experiments.