TABLE 2.
Acquired antibiotic resistance profile of S. Heidelberg isolates recovered after cecum incubation
| Isolatea | Antibiotic resistance phenotypeb | Gene(s)/locic |
|---|---|---|
| SH-2813-Parentalphe | Nal | gyrA |
| SH-2813-Parentaltyr | Nal | gyrA |
| SH-4A-0.5h-Nalphe | Nal, Cip (Inter) | gyrA |
| SH-5A-0.5h-Nalphe | Nal, Cip (Inter) | gyrA |
| SH-6A-0.5h-Naltyr | Nal, Cip | gyrA, slyA, mprA |
| SH-10A-24h-Nalphe | Nal, Cip (Inter) | gyrA, 23S-5S rRNA ITS, corA |
| SH-11A-24h-Nalphe | Nal, Cip (Inter) | gyrA |
| SH-12A-24h-Nalphe | Nal, Cip (Inter) | gyrA, 23S-5S rRNA ITS |
| SH-13A-48h-Nalphe | Nal, Cip (Inter) | gyrA, 23S 5S rRNA ITS, envZ |
| SH-14B-48h-Naltyr | Nal, Cip (Inter), Fox (Inter), Chl (Inter), Str, Smx | gyrA, slyA, mprA |
| SH-15A-48h-Naltyr | Nal, Cip (Inter) | gyrA, slyA |
| SH-13A-48h-NalAmpphe | Nal, Cip (Inter), Cro, Amp, Sam, Fox, Caz, Cfz, Cpd, Cro, Cef, Pip, Tim | gyrA, hscA, 23S ribosomal RNA, blaCMY-2 |
| SH-14A-48h-NalAmpphe | Nal, Cip (Inter), Fox, Str, Smx, Tet | gyrA, Int, 23S-5S rRNA ITS, cpxA |
| SH-15A-48h-NalAmpphe | Nal, Cip, Fox, Chl (Inter) | gyrA, Lon, 23S-5S rRNA ITS, ccmF |
| SH-15B-48h-NalAmptyr | Nal, Cip, Fox, Chl (Inter) | gyrA, dinG, slyA, 16S-23S rRNA ITS |
Parental S. Heidelberg strain was made spontaneously resistant to 200 ppm of nalidixic acid. A superscript indicates the Ser83 gyrA mutation associated with the nalidixic acid resistance carried by the isolate.
Abbreviations: Nal, nalidixic acid; Cip, ciprofloxacin; Str, streptomycin; Tet, tetracycline; Smx, sulfamethoxazole; Chl, chloramphenicol; Amc, amoxicillin-clavulanic acid; Amp, ampicillin; Sam, ampicillin-sulbactam; Fox, cefoxitin; Cro, ceftriaxone; Cfz, cefazolin; Cpd, cefpodoxime; Caz, ceftazidime; Cef, cefuroxime; Pip, piperacillin; Tim, ticarcillin-clavulanic acid; Inter, intermediate resistance.
Gene or loci that acquired a mutation. Boldface denotes antibiotic resistance gene carried on a plasmid. ITS, internal transcribed spacer.