TABLE 3.

Resistance phenotype, β-lactamases, and plasmids detected in S. Heidelberg and E. coli donor, recipient, and recombinant strains from in vitro mating experiments^a

Mating IDb	Strain ID	Resistance phenotype	AmpC gene	Plasmid(s) identified	IncK2 contig size (bp)
Solid plate
SH-2813don	SH-13A-48h-NalAmpphe	Amp, Amc, Fox, Cro, Nal	CMY-2	IncX1, IncK2	88,798 (C)
SH-2813recc	SH-14A-48h-NalAmpphe	Str, Nal	None	IncX1	NA
SH-2813don	SH-13A-48h-NalAmpphe	Amp, Amc, Fox, Cro, Nal	CMY-2	IncX1, IncK2	88,798 (C)
Ec15cecarec	Ec15ceca	Tet	None	IncFIB, Col440i	NA
SH2813_D_Ec15ceca_R	NA	Amp, Amc, Fox, Cro, Tet	CMY-2	IncK2, IncFIB, Col440i	88,178
SH2813_D_Ec15ceca_Rdon	NA	Amp, Amc, Fox, Cro, Tet	CMY-2	IncK2, IncFIB, Col440i	88,178
SH-2813rec	SH-2813-Parentaltyr	Nal	None	IncX1	NA
Ec15ceca_D_SH2813_R	NA	Amp, Amc, Fox, Cro, Nal	ND	ND	ND
Ec6BLdon	Ec6BL	Amp, Amc, Fox, Cro, Gen, Str, Smx, Tet	CMY-2	IncK2, IncF4:A5:B1, IncFII, Col (MG828)	88,889 (C)e
SH-2813rec	SH-2813-Parentaltyr	Nal	None	IncX1	NA
Ec6BL_D_SH2813_R2	NA	Gen, Str, Smx, Tet, Nal	None	IncFII, IncX1	NA
Ec6BL_D_SH2813_R	NA	Amp, Amc, Fox, Cro, Nal	CMY-2	IncK2, IncX1	85,244
Ec15BLdon	Ec15BL	Amp, Amc, Fox, Cro	CMY-2	IncK2, IncF4:A-:B1, Col (MG828)	85,509e
SH-2813rec	SH-2813-Parentaltyr	Nal	None	IncX1	NA
Ec15BL_D_SH2813_Rd	NA	Amp, Amc, Fox, Cro, Nal	CMY-2	IncK2, IncX1	84,268, 84,274, 84,273
Broth
SH-2813don	SH-13A-48h-NalAmpphe	Amp, Amc, Fox, Cro Nal	CMY-2	IncX1, IncK2	88,798 (C)
SH-2813recc	SH-2813	None	None	IncX1	NA

^aAbbreviations: ID, identifier; Gen, gentamicin; don, donor; rec, recipient; ND, not determined; NA, not applicable; C, complete circular contigs.

^bBoldface indicates recombinant strains with acquired antibiotic resistance.

^cRecipient did not acquire β-lactamase resistance from donor.

^dWGS was done on 3 individual colonies that acquired β-lactamase resistance from the donor.

^eDe novo hybrid assembly was done using Illumina short- and MinION long-read technology (see also Table S5).