FIG 7.

Growth and phenotype of 1° and 2° cells at high and low temperatures. (A) 1° cells do not recover growth after being incubated for 30 days at 4°C and already show loss of pigmentation after 25 days at 4°C. In contrast, 2° cells restart growth after 30 days of exposure to cold and are not affected at all in their fitness or phenotype. (B) 2° cells were capable of growing at 37°C when cultivated in liquid culture while growth of 1° cells was highly decreased under this condition (i and ii). Upon streaking both cell forms onto agar plates and incubating them at 37°C, only 2° cells were able to form colonies (iii). All experiments were independently performed three times. Error bars represent standard deviation.