TABLE 1.

Genes corresponding to 1° cell-specific features downregulated in 2° cells^a

Phenotype and gene	1° cells	2° cells	FC by growth phase (2° wt/1° wt)b
			Exp	Stat
Bioluminescence	+++	+
luxC			NS	−11.56
luxD			NS	−10.85
Pigmentation	+	−
antA			−19.71	−25.95
antB			−19.52	−57.15
antC			−36.69	−15.25
antD			−35.16	−13.11
antE			−20.61	NS
antF			−30.46	−26.52
antG			−20.86	NS
antH			−12.11	−10.52
antI			−12.73	−30.02
Crystal proteins	+	−
cipA			−5.01	−27.91
cipB			NS	−16.21
PluDJC_07765			−4.20	−47.76
Antibiotic production	+	−
PluDJC_04580			−11.29	NS
PluDJC_045805			−5.06	NS
PluDJC_04590			−4.90	NS
PluDJC_15990			NS	−5.47
PluDJC_16670			−5.58	NS
stlA			−4.95	NS
Cell clumping	+	−
pcfA			NS	−64.84
pcfB			NS	−87.19
pcfC			NS	−110.61
pcfD			NS	−100.05
pcfE			NS	−10.98
pcfF			NS	−10.52
Protease production	++	+
prtA			−8.47	NS
Lipase production	+	−
pdl			NS	−6.33

^aGenes were differentially transcribed between 1° and 2° cells in exponentially growing or stationary phase cultures. The presence (+) or absence (−) of the phenotype as it is described in the literature is indicated.

^bFold change (FC) was calculated as the level of expression in wild-type 2° cells/expression in wild-type 1° cells. An FC value of less than −3 or greater than 3 was considered significant (P ≤ 0.05). NS, not significant. Exp, exponential growth phase; Stat, stationary growth phase; wt, wild type.