TABLE 1.
Genes and primers used in the qRT-PCR assay
| Gene | Annotation | Primer sequence (5′–3′) |
|
|---|---|---|---|
| Forward | Reverse | ||
| Primer for the oxidoreductase gene | |||
| ZMO1116 | Oxidoreductase | TGTGGTTTGGGCCATCCG | TGTCGGTGCGTCCTGTTTGT |
| ZMO1772 | NAD(P)H quinone oxidoreductase | GGTCGCCTTGTCATTGTCG | GGCTGTTCTGGCACGCAT |
| ZMO1885 | NADH flavin oxidoreductase | TGGAGTGATGCCCAAGTAGAAG | CACTGACATTAGACGGCACCATA |
| Primer for the reductase gene | |||
| ZMO1993 | NADPH quinone reductase | GCGGTGTCGGTAGCTTGTT | GCCTTCGCCGTGATTCTG |
| ZMO0833 | UDP-N-acetylenolpyruvoylglucosamine reductase | ATCGCCTGCGTTGTGGTG | GCATTCATGCGGATCATACCA |
| ZMO1222 | 3-Oxoacyl-(acyl-carrier-protein) reductase | TTAGCCGTGCCGTCATCAGA | CGATCATGCCTGCCTTTGC |
| ZMO1303 | Pyrroline-5-carboxylate reductase | ACAACCCTGATTTCTATTCTTGCC | AACAACGCCCTTCCCTAACG |
| ZMO1984 | Aldo-keto reductase | TCGCCATTTGTCAGCCTATC | CAGCAAAGTTACTGCTACCCAC |
| ZMO1254 | Redoxin domain-containing protein | CGCTATGGTAATCCCTATCAGGC | GCTCATAAGCTGTGGCAAATCC |
| Primer for the dehydrogenase gene | |||
| ZMO1335 | NAD(P)H dehydrogenase (quinone) | CCCGATTTGGGCGTTTG | AGACCGCTCCGACCTTTCC |
| ZMO1949 | NAD(P)H dehydrogenase (quinone) | TGGAGGCAAGCGGTTCTAC | GCAACAGCGGTTTGAGCAT |
| ZMO0157 | d-Isomer-specific 2-hydroxyacid dehydrogenase | TATGGCTGTCGGGTTATTCC | TTCGGCGATGGCTTGG |
| ZMO0788 | d-Sorbitol dehydrogenase | CAGTTGTCACGGTCGCAATG | GCCGCAATCTGACTATCGTTTA |
| ZMO1576 | Short-chain dehydrogenase/reductase | TCTATCGTCGCTAAGGGTGGTC | GCTGCAATCCCATAAAGAACG |
| ZMO1696 | Zinc-binding alcohol dehydrogenase | TCCGAAGACAGGCGAAGAATG | CCTCGTCACCGACCTTAAATAGC |
| Primer for other genes | |||
| ZMO1399 | Fatty acid hydroxylase | GAAACGGATTCACTATGACCAC | CCGACAGCATAACCGATACA |
| ZMO0020 | Hypothetical protein | CCGATCTAGTAAGCCAATTCACC | TTTCAAATCTGTTGGTTGGGTGT |
| ZMO0021 | Hypothetical protein | CCACTTCATATCGCTTCTGTCG | GCGTAATCGGTGATCCCAAA |
| ZMO0074 | Hypothetical protein | CGATAAAGACCGCCCGACC | TCGCCAAGCAGGCATTCG |
| ZMO1821 | Hypothetical protein | AGAAAGCCGCCGCCATC | CACCATCATACCAACTGTCAACG |
| ZMOr003a | 16S rRNA gene | TTAAGTTGGGCACTTTAGAGGAAC | TGTCACCGCCATTGTAGCAC |
a
The 16S rRNA gene ZMOr003 was used as the internal control to normalize the difference of the data.