TABLE 1.
Genotypic characteristics of CTX-M-producing E. coli isolates recovered from WWTP A influents
| Date, no. of isolates (n = 50) | blaCTX-M gene | n | Inflow port A-1 (n = 20) |
Inflow port A-2 (n = 15) |
Inflow port A-3 (n = 15) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Phylogroup | ST | Serotype O25b | Subclone/lineage | Phylogroup | ST | Serotype O25b | Subclone/lineage | Phylogroup | ST | Serotype O25b | Subclone/lineage | |||
| September 2017, 11 | blaCTX-M-27 | 3 | B2 | ST2252a | Non-O25b | Non-H30 | B2 | ST131 | Non-O25b | Non-H30 | ||||
| F | ST648 | |||||||||||||
| blaCTX-M-55 | 2 | A | ST10a,b | F | ST648 | |||||||||
| blaCTX-M-14 | 2 | F | ST648c | C | ST167c | |||||||||
| blaCTX-M-15 | 1 | B2 | ST127 | Non-O25b | Non-H30 | |||||||||
| blaCTX-M-3 | 1 | B1 | ST4720 | |||||||||||
| blaCTX-M-1 | 1 | B1 | ST2073-likea | |||||||||||
| blaCTX-M-64 | 1 | C | ST10 | |||||||||||
| October 2017, 15 | blaCTX-M-14 | 5 | D | ST38 | D | ST38 | ||||||||
| F | ST354c | F | ST354c | |||||||||||
| F | ST648 | |||||||||||||
| blaCTX-M-15 | 3 | A | ST10 | A | ST44c | |||||||||
| D | ST405 | |||||||||||||
| blaCTX-M-3 | 2 | B1 | ST156a,c | |||||||||||
| B2 | ST127 | Non-O25b | Non-H30 | |||||||||||
| blaCTX-M-55 | 2 | B1 | ST155 | D | ST5150 | |||||||||
| blaCTX-M-27 | 2 | B2 | ST131 | Non-O25b | Non-H30 | F | ST1722 | |||||||
| blaCTX-M-2 | 1 | F | ST117 | |||||||||||
| November 2017, 12 | blaCTX-M-14 | 5 | D | ST38 | F | ST393c | A | ST10c | ||||||
| B2 | ST131a | Non-O25b | Non-H30 | |||||||||||
| F | ST10c | |||||||||||||
| blaCTX-M-15 | 4 | A | ST43 | B1 | ST4681 | |||||||||
| A | ST617 | |||||||||||||
| F | ST648 | |||||||||||||
| blaCTX-M-24 | 1 | F | ST354c | |||||||||||
| blaCTX-M-27 | 1 | B2 | ST131 | O25b | H30R/non-Rx | |||||||||
| blaCTX-M-15 and blaCTX-M-14 | 1 | F | ST648a | |||||||||||
| December 2017, 12 | blaCTX-M-15 | 4 | A | ST10 | A | ST43 | A | ST43 | ||||||
| B2 | ST131a | O25b | H30R/Rx | |||||||||||
| blaCTX-M-27 | 4 | A | ST46a | B2 | ST131a | O25b | H30R/non-Rx | D | ST69a | |||||
| B2 | ST131b | O25b | H30R/non-Rx | |||||||||||
| blaCTX-M-14 | 2 | D | ST69c | D | ST38 | |||||||||
| blaCTX-M-55 | 1 | B2 | ST676 | O25b | Non-H30 | |||||||||
| blaCTX-M-123 | 1 | F | ST1674d | |||||||||||
a
Positive for tetA gene.
b
Positive for fosA3 gene.
c
Positive for tetB gene.
d
The isolate also carried the blaCMY-2 gene.