TABLE 2.
Genotypic characteristics of CTX-M-producing E. coli isolates recovered from WWTP B, C, and D influents
| WWTP (mo yr) | No. of isolates (n = 23) | blaCTX-M gene | No. of specimens | Finding in the indicated location |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Phylogroup | ST | Serotype O25b | Subclone/lineage | Phylogroup | ST | Serotype O25b | Subclone/lineage | ||||
| B (July 2018) | 7 | Inflow port B-1 (n = 5) | Sampling pointa B-2 (n = 2) | ||||||||
| blaCTX-M-55 | 2 | B1 | 58b | ||||||||
| E clade | 10b,c | ||||||||||
| blaCTX-M-14 | 1 | B2 | 131b | Non-O25b | Non-H30 | ||||||
| blaCTX-M-15 | 1 | D | 70e | ||||||||
| blaCTX-M-27 | 2 | B2 | 131 | O25b | H30R/non-Rx | B2 | 131b | O25b | H30R/non-Rx | ||
| blaCTX-M-174 | 1 | B2 | 131b | O25b | H30R/non-Rx | ||||||
| C (August 2018) | 4 | Inflow port C-1 (n = 2) | Sampling pointa C-2 (n = 2) | ||||||||
| blaCTX-M-14 | 2 | B2 | 131 | Non-O25b | Non-H30 | ||||||
| D | 38 | ||||||||||
| blaCTX-M-15 | 1 | F | 648 | ||||||||
| blaCTX-M-65 | 1 | B1 | 2179 | ||||||||
| D (September 2018) | 12 | Inflow port D-1 (n= 6) | Inflow port D-2 (n = 6) | ||||||||
| blaCTX-M-27 | 5 | B2 | 131b | Non-O25b | Non-H30 | B2 | 131 | Non-O25b | Non-H30 | ||
| B2 | 131b | O25b | H30R/non-Rx | B2 | 131 | O25b | H30R/non-Rx | ||||
| D | 2003 | ||||||||||
| blaCTX-M-14 | 4 | D | 69d | D | 38 | ||||||
| B1 | 58b | ||||||||||
| A | 206b | ||||||||||
| blaCTX-M-8 | 1 | B1 | 345 | ||||||||
| blaCTX-M-15 | 1 | D | 69b | ||||||||
| blaCTX-M-55 | 1 | B1 | 767b,c | ||||||||
a
The sample contained inflow water and return flows from sludge treatment.
b
Positive for tetA gene.
c
Positive for fosA3 gene.
d
Positive for tetB gene.
e
The isolate also carried the blaDHA-1 gene.