Genome comparison between CDFM21L.1 and CBS 12357T. (A) Translocations in CDFM21L.1 relative to CBS 12357T. The diagram in the first panel shows a magnification of the right end of ChrV, the entire ChrVI, and the left end of ChrVII, and it displays the paracentric inversion of the subtelomeric region of ChrVII left arm (approximately 8 kbp). The second panel represents the concatenated whole-genome alignment of S. eubayanus strains CBS 12357 and CDFM21L.1. The red vertical lines indicate the chromosome separations. The third panel shows, on the left, a magnification of a translocation of approximately 12 kbp from the left subtelomeric region of chromosome VIII to the right subtelomeric region of chromosome VI and a reciprocal translocation between approximately 20 kbp from the right subtelomeric region of chromosome V and approximately 60 kbp from the center of the chromosome. Genome synteny is indicated with colored blocks. (B) Relative chromosome position of gene presence differences between CDFM21L.1 (blue) and CBS 12357T (red). (C) Representation of the assembled CDFM21L.1 S. eubayanus chromosomes. The black boxes denote newly added sequences. New annotated open reading frames and gene entries modified relative to the CBS 12357T draft genome are shown (13).