FIG 7.

Hybridization of maltotriose-deficient S. cerevisiae and S. eubayanus strains leading to cross talk restoring maltotriose utilization explains the S. pastorianus phenotype. (A) Characterization of S. cerevisiae CBC-1 (▼), S. eubayanus CDFM21L.1 (▲), and hybrid HTSH020 (▪) on mock wort at 12°C. Consumption of maltose (red) and maltotriose (blue) and production of ethanol (green) were measured from the supernatant by HPLC. Data represent averages and standard deviations from biological triplicates. (B) Normalized transcript levels of maltose metabolism genes from HTSH020 mid-exponential phase grown on glucose (red), maltose (blue), and maltotriose (green) at 20°C were calculated from duplicate RNA sequencing experiments (2 by 150 bp) using the FPKM method. All data represent averages and standard deviations of two biological duplicates.