TABLE 1.
Saccharomyces strains used in this study
| Name | Species | Relevant genotypea | Source or reference |
|---|---|---|---|
| CDFM21L.1 | S. eubayanus | Wild type, Mal− Mtt− | 35 |
| ABFM5L.1 | S. eubayanus | Wild type, Mal− Mtt− | 35 |
| CBS 12357 | S. eubayanus | Wild type, Mal+ Mtt− | 1; Westerdijk Institutec |
| CBS 1483 | S. pastorianus | Wild type, Mal+ Mtt+ | Westerdijk Institute |
| CEN.PK113-7D | S. cerevisiae | MATa MAL1x MAL2x MAL3x MAL4x MAL2-8C SUC2 LEU2 URA3 | 84 |
| IMZ616 | S. cerevisiae | MATa ura3-52 LEU2 MAL2-8C malΔ mp2/3hΔ suc2Δ ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ pUDC156 (Spcas9 URA3 ARS4 CEN6) | 41 |
| IMX1365 | S. cerevisiae | MATa ura3-52 LEU2 MAL2-8C malΔ mph2/3Δ suc2Δ ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ pUDC156 (URA3 cas9) sga1Δ::ScTDH3p-ScMAL12-ScADH1t ScTEF1p-ScAGT1-ScCYC1t | 13 |
| IMX1702 | S. cerevisiae | MATa ura3-52 LEU2 MAL2-8C malΔ mph2/3Δ suc2Δ ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ pUDC156 (URA3 cas9) sga1Δ::ScTDH3p-ScMAL12-ScADH1t ScTEF1p-SeMALT1-ScCYC1t | This study |
| IMX1704 | S. cerevisiae | MATa ura3-52 LEU2 MAL2-8C malΔ mph2/3Δ suc2Δ ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ pUDC156 (URA3 cas9) sga1Δ::ScTDH3p-ScMAL12-ScADH1t ScTEF1p-SeMALT2-ScCYC1t | This study |
| IMX1706 | S. cerevisiae | MATa ura3-52 LEU2 MAL2-8C malΔ mph2/3Δ suc2Δ ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ pUDC156 (URA3 cas9) sga1Δ::ScTDH3p-ScMAL12-ScADH1t ScTEF1p-SeMALT3-ScCYC1t | This study |
| IMX 1708 | S. cerevisiae | MATa ura3-52 LEU2 MAL2-8C malΔ mph2/3Δ suc2Δ ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ pUDC156 (URA3 cas9) sga1Δ::ScTDH3p-ScMAL12-ScADH1t ScTEF1p-SeAGT1-ScCYC1t | This study |
| IMX1313 | S. cerevisiae | MATa ura3-52 LEU2 MAL2-8C malΔ mph2/3Δ suc2Δ ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ sga1Δ::ScTEF1p-ScMAL31-ScCYC1t pUDC156 (URA3 cas9) | This study |
| IMX1313Δ | S. cerevisiae | MATa ura3-52 LEU2 MAL2-8C malΔ mph2/3Δ suc2Δ ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ sga1Δ::ScTEF1p-ScMAL31-ScCYC1t | This study |
| IMZ752 | S. cerevisiae | MATa ura3-52 LEU2 MAL2-8C malΔ mph2/3Δ suc2Δ ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ sga1Δ::ScTEF1p-ScMAL31-ScCYC1t pUDE843 (ori [ColE1] bla 2μ ScTDH3p-SeMALS1-ScADH1t URA3) | This study |
| IMZ753 | S. cerevisiae | MATa ura3-52 LEU2 MAL2-8C malΔ mph2/3Δ suc2Δ ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ sga1Δ::ScTEF1p-ScMAL31-ScCYC1t pUDE844 (ori [ColE1] bla 2μ ScTDH3p-SeMALS2-ScADH1t URA3) | This study |
| IMK820b | S. eubayanus | MATa/MATα Semalt1Δ/Semalt1Δ | This study |
| IMK823b | S. eubayanus | MATa/MATα Seagt1Δ/Seagt1Δ (X3) | This study |
| IMX1939b | S. eubayanus | MATa/MATα Semalt1Δ/Semalt1Δ Sesga1Δ::ScMAL13/Sesga1Δ::ScMAL13 | This study |
| IMX1940b | S. eubayanus | MATa/MATα Seagt1Δ/Seagt1Δ Sesga1Δ::ScMAL13/Sesga1Δ::ScMAL13 | This study |
| IMX1762b | S. eubayanus | MATa/MATα Sesga1Δ::ScMAL12/Sesga1Δ::ScMAL12 | This study |
| IMX1765b | S. eubayanus | MATa/MATα Sesga1Δ::ScMAL13/Sesga1Δ::ScMAL13 | This study |
| CBC-1 | S. cerevisiae | MATa/MATα Mal+ Mtt− | Lallemand |
| HTSH020 | S. cerevisiae × S. eubayanus | MATa/MATα Mal+ Mtt+ | This study |
a
The abbreviation malΔ indicates mal11-mal12::loxP mal21-mal22::loxP mal31-32::loxP. Mal and Mtt denote the maltose and maltotriose phenotype, respectively.
b
Direct derivatives of the wild-type S. eubayanus strain CDFM21L.1.
cWesterdijk Fungal Biodiversity Institute (www.westerdijkinstitute.nl/).