FIG 2.

RT-qPCR transcriptional analysis of genes that may be regulated by AcrR under ethanol stress. (A) Transcriptional levels of genes that may be regulated by AcrR in WT, ΔacrR, and P-acrR strains under 9% (vol/vol) ethanol. (B) Transcriptional levels of genes that may be regulated by AcrR in the WT⁺ and acrR overexpression (acrR⁺) strains under 9% (vol/vol) ethanol. Relative values were obtained using the transcription of gyrB as reference. cDNA used as template in this experiment was diluted 1,000-fold. The x axis represents different genes. Values and standard deviations were calculated from three repeated samples. **, P < 0.01; *, P < 0.05.