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. 2019 Oct 30;85(22):e01690-19. doi: 10.1128/AEM.01690-19

FIG 5.

FIG 5

Organization and transcription unit of fab gene cluster in L. plantarum NF92. (A) Organization of the fab gene cluster in L. plantarum NF92. The gene cluster was divided into two parts (red lines) for RT-PCR to determine potential cotranscription. fabZ1, 3-hydroxyacyl-ACP dehydratase FabZ; fabH2, ketoacyl-ACP synthase III; acpP, acyl carrier protein; fabD, ACP S-malonyltransferase; fabG1, SDR family oxidoreductase; fabF, β-ketoacyl-[acyl-carrier-protein] synthase II; accB2, acetyl-CoA carboxylase biotin carboxyl carrier protein subunit; fabZ2, β-hydroxyacyl-ACP dehydratase; accC1, acetyl-CoA carboxylase biotin carboxylase subunit; accD1, acetyl-CoA carboxylase carboxyl transferase subunit beta; accA1, acetyl-CoA carboxylase carboxyl transferase subunit alpha; fabI, enoyl-[acyl-carrier-protein] reductase FabI; sfp, 4′-phosphopantetheinyl transferase superfamily protein. (B) RT-PCR for cotranscription analysis of genes in fab gene cluster. fabZ1-accC1, 5,354 bp; accC1 to sfp, 2,610 bp. RNA was extracted from the WT strain. Lanes: G, positive controls with genomic DNA; RT, cDNA from the RNA sample; NC, negative controls consisting of DNase I-treated RNA sample; M, DNA marker.