TABLE 1.
Occurrence of Gram-negative Enterobacterales strains, nonfermenting organisms, and PFGE type 00531 ESBL-producing K. oxytoca isolates in environmental samples obtained during on-site inspection of risk areas
Room | Sampling point | Counts (CFU/ml or total Gram-negative bacterial count) | Microbiological differentiation | CTX-M | PFGE type 00531/ST201 K. oxytoca |
---|---|---|---|---|---|
Nursing care room, storage rooms | Working basin, siphon | 2.1 × 105 | Serratia marcescens | ||
Hand wash basin, siphon water | 1.6 × 106 | K. oxytoca | Positive | Yes | |
Raoultella terrigena | Negative | ||||
Enterobacter cloacae complex | Negative | ||||
Hand wash basin, drainage hole cover | Scattered/swab (no CFU/ml) | E. cloacae complex | Negative | ||
K. pneumoniae | Negative | ||||
Staff toilet | Hand wash basin, fresh water | Not evaluable/100 ml | Gram-negative nonfermenting rods | ||
Hand wash basin, siphon water | 1.5 × 104 | P. aeruginosa, S. maltophilia | |||
Toilet, water | 1.0 | S. maltophilia | |||
Staff room | Kitchen sink, siphon water | 3.0 × 105 | K. oxytoca | Positive | Yes |
P. aeruginosa | Negative | ||||
Water reservoir tank, espresso machine | 10.0 CFU/50 ml | S. maltophilia | |||
Hygiene sluice, PNC | Hand wash basin, siphon water | 1.3 × 104 | P. aeruginosa, Gram-negative nonfermenting rods | ||
Basement, laundry room | Tumble dryer, water tray | Not evaluable/50 ml | Gram-negative nonfermenting rods | ||
Washing machine 1, residual water, rubber mantle | 3.9 × 102 | K. oxytoca | Positive | Yes | |
Washing machine 1, detergent compartment | Not evaluable/swab | K. oxytoca | Positive | Yes | |
P. aeruginosa | Negative | ||||
Washing machine 2, detergent compartment | Not evaluable/swab | P. aeruginosa | |||
Laundry | Hat 1 | >109 | K. oxytoca | Positive | Yes |
Hat 2 | >109 | K. oxytoca | Positive | Yes | |
Hat 3 | >109 | K. oxytoca | Positive | Yes | |
Hat 4 | >109 | K. oxytoca | Positive | Yes | |
Socks 1 | >109 | K. oxytoca | Positive | Yes | |
Socks 2 | >109 | K. oxytoca | Positive | Yes |