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. 2019 Jul 5;47(16):8720–8733. doi: 10.1093/nar/gkz559

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — A subset of NSUN2 protein localizes in the mitochondria. (A, B) Immunofluorescence labelling of a HA-tagged NSUN2 construct (green) in osteosarcoma 143B cells. Cells were counterstained for the mitochondrial import receptor subunit TOM20 (red) and DAPI (blue). Scale bar: 10 μM. NSUN2 localizes mainly to the nucleus (A). A fraction of the NSUN2 protein co-localizes with the mitochondria (B). (C) Sub-cellular localization of a FLAG-tagged NSUN2 construct analysed by western blotting with antibodies against FLAG, TOM22 (mitochondrial outer membrane), mtSSB1 (mitochondrial matrix) and GAPDH (cytosol). HEK293T cells were fractionated into debris (‘D’), cytosol (‘C’) and mitochondria. ‘T’ indicates the total cell lysate.