A, RNA-Seq analysis can be used as additional support for DNA aberrations when a single mutated gene is itself highly expressed or downstream genes are highly expressed as a result of the mutation. B, With multiple mutated genes, RNA-Seq analysis can be used to prioritize among them based on high expression of the mutated gene itself or downstream targets. C, If DNA aberration is not expressed, nor are downstream genes, RNA-Seq analysis can be used to deprioritize DNA abnormalities with no evidence of effectiveness at the level of RNA. D, RNA-Seq analysis can reveal an abnormality in the absence of DNA mutation.