Table 2. Bacterial Contact Killing of Gram-Positive Bacterial Strains of Hyperbranched QAC Coatings on PDMS, Carried Out with a Petrifilm Assaya.
| bacterial challenge CFU/sample |
|||||
|---|---|---|---|---|---|
| no. | sample | 102 | 103 | 104 | % reduction |
| S. epidermidis ATCC 12228 | |||||
| 1 | control (PDMS) | 69 ± 4 | b | b | b |
| 1a | HBP-PEI+ coating | 0 | 0 | 0 | 100 |
| S. epidermidis 308 | |||||
| 2 | control (PDMS) | 65 ± 7 | b | b | |
| 2a | HBP-PEI+ | 0 | 0 | 4 ± 1.5 | 100 |
| S. aureus ATCC 12600 | |||||
| 3 | control (PDMS) | 101 | b | b | b |
| 3a | HBP-PEI+ coating | 7 | b | 11 | 99.9 |
| Streptococcus salivarius GB 24/9 | |||||
| 4 | control (PDMS) | 77 ± 6 | b | b | b |
| 4a | HBP-PEI+ | 4 ± 2 | 12 ± 2 | 30 ± 5 | 99.7 |
| 4b | HBP-PEI+ + SCFc | 1 ± 1 | 8 ± 7 | b | 99.2 |
| Rothia dentocariosa GBJ 52/2B | |||||
| 5 | control (PDMS) | 85 | b | b | b |
| 5a | HBP-PEI+ | 9 ± 12 | 20 ± 7 | 33 ± 11 | 99.7 |
| 5b | HBP-PEI+ + SCFc | 1 ± 1 | 3 ± 1 | b | 99.7 |
a
All experiments were performed in triplicate.
b
Not measured.
c
SCF, in the presence of a salivary conditioning film.