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. Author manuscript; available in PMC: 2019 Oct 31.
Published in final edited form as: J Vis Exp. 2019 Sep 11;(151):10.3791/58645. doi: 10.3791/58645

Figure 2: Schematic view of the experimental design for the dual reconstitution of humanized liver and immune system mice, followed by HIV-1 infection.

Figure 2:

TK-NOG mice are injected with ganciclovir (GCV) at a dose of 6 mg/kg, 2x a day, on day −7 and day −5, followed by a treosulfan injection on days −3, −2 and −1. To screen the mice for the transplantation (Tx), an alanine aminotransferase (ALT) assay is performed one day before the surgery, and mice with ALT levels of >200 and <600 U/L are selected. After transplantation, the mice are checked for a reconstitution of the human immune system by flow cytometry (FACS) and for liver reconstitution by assessing their albumin level using ELISA. The mice are infected with HIV-1 5 weeks before they are sacrificed.