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. Author manuscript; available in PMC: 2019 Oct 31.
Published in final edited form as: J Vis Exp. 2019 Sep 11;(151):10.3791/58645. doi: 10.3791/58645

Figure 3: Flow cytometry analysis gating strategy for the human cell distribution of blood.

Figure 3:

(A) First, lymphocytes are gated on whole blood based on FSC-A and SSC-A. (B) Single cells are gated on lymphocytes. (C) Human CD45+ leukocytes are gated on single cells using mouse CD45 and human CD45. (D) CD3+ T cells and CD19+ B cells are identified on gated CD45+ human leukocytes. (E) CD4+ T helper cells and CD8+ cytotoxic T cells are identified in gated CD3+ T cells. (F) CD14+ monocytes are gated from human CD45+ leukocytes. The results represented here are from one mouse transplanted with dual human hepatocytes and HSPCs.