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. 2019 Aug 5;28:73–82. doi: 10.1016/j.molmet.2019.08.002

Figure 1.

Figure 1

Beta3-adrenergic stimulation enhances Tregs in humanized mice. A) Gene expression analysis of thermogenic markers in murine VAT after vehicle or mirabegron treatment in vivo. B) Representative flow cytometric analysis of ex vivo Tregs in humanized mice after vehicle or mirabegron treatment in vivo. Tregs are gated as CD8A−CD11B−CD14−CD19−eF450−CD45+CD3+CD4+CD127lowCD25hi. C) Summary plot of (B) with 5 mice per group from two independent experiments. D) Representative flow cytometric analysis of in vitro induced Tregs isolated from humanized mice after in vivo treatment with vehicle or mirabegron. Tregs are gated as live CD3+CD4+CD127lowCD25hiFOXP3hi. E) Summary plot of (D). F) Dose-response titration of mirabegron administered during human in vitro Treg induction. Two independent experiments. * = p < 0.05, ** = p < 0.01, *** = p < 0.01, **** = p < 0.001; one-way ANOVA with mirabegron vs. vehicle.