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Short-term cold exposure in vivo improves human Treg induction in vitro. A) Representative flow cytometric analysis of in vitro induced Tregs before (T0) and after (T2) in vivo cold exposure. B) Paired analyses of (A). Each dot represents the mean of 3–4 replicates and an individual experiment performed with paired samples. C) Fold change of in vitro induced Tregs normalized to individual T0. * = p < 0.05, ** = p < 0.01, *** = p < 0.01, **** = p < 0.001; paired t-test.
