Figure 1.

Transduction of Human HSPCs and Cell Lines by AAV6 and AAV9

(A) HSPCs were transduced with AAV6 or AAV9 vectors containing a CMV-GFP cassette at indicated MOIs, and GFP expression was measured 2 days later by flow cytometry, for n = 2 HSPC donors. Data are indicated as mean ± SEM. (B) HEK293T, HeLa, and K562 cells were transduced with AAV6 or AAV9 CMV-GFP vectors at an MOI of 10⁵, and GFP expression was measured 2 days later by flow cytometry. (C) HEK293T cells were transduced with AAV6 or AAV9 CMV-GFP vectors at an MOI of 5 × 10⁵, and GFP expression was measured at day 2 and day 17 by flow cytometry.