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. 2019 Jul 15;27(10):1737–1748. doi: 10.1016/j.ymthe.2019.07.002

Figure 2.

Figure 2

BGas Repression Results in Increased Expression of CFTR

(A) Screen panel of ten gapmers. (B) Top, diagram of CFTR and BGas showing gapmer targeting BGas intron 1, bottom. (C) Directional RT-PCR (inverted picture of an end-point PCR) of BGas and (D) relative densitometric analysis of the individual band in nasal cells from a CF patient with F508del. (E and F) Gapmer-Tat CPP increased CFTR expression in (E) nasal cells from healthy donor-WT and (F) in nasal cells from a CF patient with F508del mutation. CFTR expression was determined by qRT-PCR. (G and H) CFTR ELISA showing CFTR protein level expression increased after being treated with BGas-gapmer in (G) nasal cells from healthy donor-WT and (H) nasal cells from a CF patient with F508del mutation. For directional RT-real-time qPCR (C) and ELISA (G and H), experiments were performed in triplicate in cells shown with the SEMs and p values from a paired two-sided t test, *p = 0.001, **p < 0.05.