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. 2019 Jul 12;27(10):1848–1862. doi: 10.1016/j.ymthe.2019.06.017

Figure 7.

Figure 7

R-Tf-D-LP4 Peptide Treatment Increased Carbohydrate Metabolism in HFD-32 Mice at the NASH Stage

(A) qRT-PCR analysis of mRNA levels of Glut-1, Glut-2, and Glut-4 in chow-fed mice (black bars) and HFD-32-fed mice untreated (dark gray bars) or treated with R-Tf-D-LP4 (light gray bars). Immunoblot (B) and quantitative analysis (C) of metabolism-related proteins and SIRT1 and SIRT6. (D) Immunoblot of phosphorylated AMPK (p-AMPK), with the relative band intensity presented in relative units (RUs) (n = 3). β-actin served as an internal loading control. Results are means ± SEM (n = 3–5; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001).