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. 2019 Jul 5;28:144–150. doi: 10.1016/j.molmet.2019.07.003

Figure 4.

Figure 4

Islet function is increased 84 days after VSG. (A) Glucose stimulated insulin secretion, and (B) calculated insulin secretion rate dose response curve from ex vivo perifusion of islets isolated for PF-Sham animals (n = 6) and VSG animals (n = 8) treated with 2, 8, 12, and 16 mM glucose; (C) EC50 and (D) peak insulin secretion rate calculated from the dose response curve. (E) Representative Ca2+ traces in islets from PF-Sham (totaling 92 islets from 3 mice) or VSG animals (totaling 93 islets from 3 mice), (F) percentage of islets undergoing active Ca2+ oscillations when treated with either 7 mM glucose (7G) or 10 mM glucose (10G). Quantification of (G) calcium plateau fraction (the fraction of time spent in the active state of each oscillation, defined as >50% oscillation amplitude) and (H) oscillation period. PF-Sham animals are shown with a solid grey line or white/black bars; VSG animals are shown with a dashed red line or red bars. Data represent mean ± SEM; * represents p < 0.05 between PF-Sham and VSG groups; **** represents p < 0.0001 between PF-Sham and VSG groups.