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. 2019 Aug 5;28:1–13. doi: 10.1016/j.molmet.2019.08.001

Figure 6.

Figure 6

M-indAktDKO mice display a fiber type specific effect on glucose uptake. (A–B) Ex vivo insulin-stimulated 2-deoxyglucose uptake in EDL (A) or soleus (B) from M-indControl and M-indAktDKO mice with/without pretreatment of wortmannin (1 μM) (n = 20 for control and insulin, n = 4 for wortmannin). (C) Western blot of Glut1 and Glut4 protein and HSP90 in Plasma membrane fraction of hind limb and whole cell lysate of gastrocnemius muscles from M-indControl and M-indAktDKO mice stimulated with insulin (2 U/kg) for 20 min after overnight fast. (D) Western Blot analysis of phosphorylated AS160, TBC1D1 and HSP90 in gastrocnemius muscle from M-indControl and M-indAktDKO mice fasted overnight and treated with BSA or insulin (2 U/kg) for 20 min (*p < 0.05, **p < 0.01, ****p < 0.0001 vs. control; #p < 0.05 vs control insulin injected).