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31P NMR spectra showing the differential hydrolysis of 1 by variants of PTE. (a) 31P NMR spectrum of compound 1 (2 mM). SP-1 resonates at −1.43 ppm, while RP-1 resonates at −1.54 ppm. (b) Hydrolysis of 1 by 3 μM PTE-G60A. The reaction is ∼28% complete. The single phosphorus-containing product resonates at 2.05 ppm. (c) Reaction from spectrum b at ∼60% completion. (d) Hydrolysis of 1 by 0.1 μM PTE-In1W. The reaction is ∼38% complete. (e) Reaction from spectrum d at ∼50% completion.
