Figure 1.

Identification of Regulatory Elements in the CFTR 5′ UTR

(A) Structure prediction of the human CFTR 5′ UTR. Structures of potential interest are boxed in green; the uAUG site is boxed in blue. Red arrows indicate regions of overlap with the ASO screen. (B) Schematic of reporter construct. CMV, CMV promoter; HSV TK, HSV TK promoter. (C) Representative reporter assay data in three cell lines for various mutations introduced into the CFTR 5′ UTR. Data are mean + SEM. *p < 0.05, **p < 0.005 by Holm-Sidak method, α = 0.05.