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. 2019 Oct 30;50:87. doi: 10.1186/s13567-019-0707-2

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ML and MCC tree of the HA (A) and NA (B) segments. The ML trees were constructed using the GTR + gamma nucleotide substitution model with 1000 bootstrap replications (A: HA; B: NA). The MCC trees were reconstructed using the HKY + gamma nucleotide substitution model and lognormal relaxed clock. The skyline coalescent model length chain was set at 1 × 10⁸ in length and resampled every 1 × 10⁴ steps (C: HA; D: NA).