Fig. 5.

Relative expression levels of selected genes in salt-treated cotton seedlings. RNA was extracted from VIGS and TRV2 cotton seedlings leaves, and gene expression levels were measured by RT-qPCR normalized against the GhUBQ7 gene. Relative expression levels are shown for a. RBOHB, b. RBOHD, c. P5CS, d. NHX7, e. RD22, f. SOD, g. CAT, h. POD, i. MYB5, j. WRKY46, k. NAC29, l. ERF62. Mock, without salt treatment, NaCl: 250 mM NaCl treatment. *, p < 0.05 and **, p < 0.01 by Student’s t-test compared with untreated (Mock) or salt-treated TRV2 seedlings. Data represent means ± SD