Figure 5.

CAY suppresses T0901317 effect on cell viability and caspases 3/7 activation. hMSC were pretreated during 16 h with T0901317 and treated 48 h with T0901317, CAY 25 μM in the absence (white column) or presence of Palm 0.25 mM (gray column). Cell death (A) was quantified by nuclear staining with Hoechst and propidium iodide. Values are mean ± SEM of 4 individual experiments. Caspases-3/7 activity (B) was measured using the Caspases-3/7 Glo assay. Values are expressed relative to Ctrl and are mean ± SEM of 3 individual experiments. ^*p < 0.05; ^**p < 0.01 vs. Ctrl; ^#p < 0.05; ^##p< 0.01 vs. Palm 0.25 mM; ^$p < 0.05; ^$$p < 0.01 vs. Palm 0.25 mM + T0901317 100 nM.