Fig. 6.

Lmcd1 increases S6 and AKT phosphorylation as well as calcineurin expression while silencing of Lmcd1 has the opposite effect. a Representative western blot for AKT Ser⁴⁷³ phosphorylation, total AKT, S6 Ser²³⁵ phosphorylation, total S6, 4E-BP1 Thr³⁷phosphorylation, and total 4E-BP1, normalized for α-tubulin expression, in differentiated primary mouse myotubes 2 days after transduction with GFP (control) or LMCD1 adenovirus (n = 8). b Representative Western blot for calcineurin A and calcineurin B, normalized for α-tubulin expression, in differentiated myotubes transduced as in (a) (n = 8). c Representative western blot for PTEN normalized for α-tubulin expression in differentiated myotubes transduced as in (a) (n = 6). d Representative Western blot for insulin receptor substrate 1 (IRS1) Tyr⁶¹² phosphorylation normalized for α-tubulin expression in differentiated myotubes transduced as in (a) (n = 4). e Representative Western blot for AKT phosphorylation Ser⁴⁷³, total AKT, S6 phosphorylation Ser²³⁵, and total S6, normalized for α-tubulin expression, in differentiated primary mouse myotubes after 2 days transduction of scrambled shRNA or shLmcd1 adenovirus (n = 8). f Representative western blot for calcineurin A and calcineurin B, normalized for α-tubulin expression, in differentiated primary mouse myotubes transduced as in (e) (n = 8)