Fig. 7.

LMCD1 depends on calcineurin signaling to increase protein synthesis, force and calcium handling. a Gastrocnemius mass normalized by body weight after 7 days of GFP (control) or Lmcd1 expression and cyclosporine A (CsA) i.p. injection (n = 6). b In vivo muscle protein synthesis determination by puromycin incorporation in mice treated as in (a), and respective quantification (n = 6). c Force normalized for the cross-sectional area (specific force) for the different frequency tested (15–150 Hz) at 1-min interval in mice treated as in (a) (n = 10). d Myoplasmic free Ca²⁺ concentration for the different frequency tested (15–150 Hz) at 1-min interval in mice treated as in (a) (n = 10). e Specific force and myoplasmic free Ca²⁺ concentration for the mean of the fibers for the different frequencies tested (15–150 Hz) at 1-min intervals. Mice treated as in (a) (n = 10). f Percentage of force relative to the first contraction. Peak force was measured at 70 Hz tetani of 350 ms duration given at 2-s intervals for 50 contractions in mice treated as in (a) (n = 10). g Percentage of myoplasic free Ca²⁺ relative to the first contraction. Peak myoplasmic free Ca²⁺ concentration was measured at 70 Hz tetani of 350-ms duration given at 2-s intervals for 50 contractions in mice treated as in (a) (n = 10). Data is shown as mean ± SEM and *p < 0.05