Fig. 3.
Inhibition of CD147 decreases the deployment and proinflammatory activation of Ly-6C+ monocytes/macrophages (MMs) in the post-ischemic brain 72 h after tMCAO. a Representative dot plots illustrate the flow cytometry gating strategy for identification of immune cell subsets in the brain. b Flow cytometric quantification of Ly-6Chigh MMs in the ischemic and non-ischemic (sham) hemispheres from the indicated groups of mice (n = 5 mice/group). c Flow cytometric quantification of Ly-6Chigh MMs in the ischemic and non-ischemic (sham) hemispheres from the following groups of mice (n = 6/group): Spx + tMCAO, sham-Spx + tMCAO. *p < 0.05. Splenectomy (Spx) and sham-Spx surgery were performed 2 weeks prior to tMCAO. d RT-qPCR analysis of mRNA levels of M1 markers (iNOS, IL-6) and M2 marker (Arg1) detected in the FACS sorted brain microglia/macrophages from the indicated groups of mice (n = 6/groups). *P < 0.05