Table 3.

Comparison of EV Characterizing and Analyzing Techniques

Technique	DLS	NTA	nanoFACS
Feature	Techniques based on the Brownian motion of and light scattering from the particle		Techniques based on the dual forward scatter and single forward scatter
	Measure the diffusion coefficient and derive the size from that diffusion coefficient. So both are influenced by shape in the same manner		Enhanced Forward Scatter (eFSC) technology
	Ensemble measurement provides an intensity-based distribution	Particle-by-particle measurement provides a number-based distribution	Expression of certain EV surface epitopes which can be stained by specific fluorescently labeled antibodies
	Can provide the % by intensity of particles	Can give the % by number of particles	Valuable tool to study cell-type specific surface proteins or activation markers on exosomes.
	To measure particles smaller than 10 nm or larger than micron	Can often provide higher resolution,	To gain insights about EV populations originating from a particular cell type