Figure 3. The interaction between GAS5 and IKK in SV40 MES-13 cells.

(A) RNA-pull down assay using in vitro-synthesized biotinylated GAS5. Precipitation reactions were conducted using streptavidin beads and then subjected to Western blotting. (B) Protein lysates were prepared and immunoprecipitated with IKK antibody or IgG. The RNA levels of GAS5 in immunoprecipitates were measured by real-time PCR. ** P<0.01, compared with IgG.