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. 2019 Oct 18;39(10):BSR20181740. doi: 10.1042/BSR20181740

Figure 4. GAS5 regulated the expression of IKK and the activity of NF-κB.

Figure 4

(A) SV40 MES-13 cells were transfected with pcDNA or pcDNA-GAS5. Then, SV40 MES-13 cells were divided into NG group, HG group, HG+pcDNA group, HG+pcDNA-GAS5 group. The expression of IKK and the luciferase activity of NF-κB were detected in NG group, HG group, HG+pcDNA group, HG+pcDNA-GAS5 group. (B) SV40 MES-13 cells were transfected with si-control or si-GAS5. The expression of IKK and the luciferase activity of NF-κB were detected in si-control and si-GAS5 groups. (C) SV40 MES-13 cells were transfected with si-control or si-GAS5, then treated with Cycloheximide (CHX) for 0, 3, 6 and 9 h. The protein level of IKK was detected in each group. ** P<0.01, compared with NG or si-control. ##P<0.01, compared with HG+pcDNA.