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. 2019 Oct 18;39(10):BSR20191255. doi: 10.1042/BSR20191255

Figure 4. miRNA-103 direct targeted 3′UTR of SPHK1.

Figure 4

(A) A putative miRNA-103 target site in the 3′-UTR of SPHK1 mRNA was predicted by using bioinformatics analysis. (B) Wild-type (SPHK1-WT) or mutant (SPHK1-Mut) luciferase reporter and/or miRNA-103 mimic were co-transfected into HEK293T cells to measure the luciferase activity. (C) The cells were treated as indicated, the mRNA level of SPHK1 was measured by RT-PCR. (D) The cells were treated as indicated, the protein level of SPHK1 was measured by Western blotting. **, P<0.01.