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. 2019 Oct 18;39(10):BSR20191255. doi: 10.1042/BSR20191255

Figure 5. SPHK1 was essential to miRNA-103 mimic-mediated AKT inhibition and apoptosis.

Figure 5

(A,B) The chondrocytes were treated with miRNA-103 mimic or SPHK1 overexpression vector and indicated protein expressions were measured by Western blotting. (C) Percentage of apoptotic cells was measured by flow cytometry after treatment with miRNA-103 mimic and/or SPHK1 overexpression. (D) Cell proliferation was measured by CCK-8 assay. (E) Percentage of wound closure was measured by scratch wound assay. **, P<0.01; *, P<0.05.