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. 2020 Apr;31(1):1–6. doi: 10.7171/jbt.20-3101-001

FIGURE 2.

FIGURE 2

Validation of optimized protocol (A1B1C1) in breast FFPE specimens. A) Quality and quantity. B) Fragment analysis. C) PCR amplification of Pim1 kinase and GAPDH of DNA isolated from breast FFPE specimens. D) Sanger’s sequencing (chromatogram) showing deletion of adenine (A) and guanine (G) at position 6020–6021 of Exon 4 region of Pim1 kinase. Data shown are from 3 independent experiments. Quality and quantity analysis of extracted DNA are represented as means ± sd.