Table 1. Effect of M-antibodies on ERK phosphorylation in cell lines expressing human and rat Trk receptors.
| BDNF | NT-3 | M1 | M2 | M3 | M4 | M5 | M6 | M7 | ||
|---|---|---|---|---|---|---|---|---|---|---|
| TrkB (human) HEK293 | EC50 nM | 0.68 ± 0.16 (16) | 2.05 ± 0.92 (8) | >100 (4) | >100 (4) | 0.33 ± 0.08 (4) | 0.41 ± 0.19 (5) | 0.66 ± 0.22 (5) | 0.57 ± 0.12 (4) | >100 (4) |
| Max (%) | 114 ± 5.25 | 58.5 ± 5.46 | NA | NA | 89.4 ± 16.0 | 68.1 ± 11.0 | 68.7 ± 13.0 | 94.7 ± 18.0 | NA | |
| TrkB (rat) HEK293 | EC50 nM | 0.19 ± 0.03 (28) | 10.7 ± 3.47 (12) | ND | ND | 0.18± 0.05 (11) | 0.93 (2) | 0.49 (2) | 1.77 ± 0.63 (4) | ND |
| Max (%) | 104 ± 2.79 | 83.0± 17.3 | ND | ND | 70.4 ± 26.6 | 61.2 | 49.5 | 79.5 ± 2.14 | ND | |
| TrkC (human) NIH3T3 | EC50 nM | 26.8 ± 10.4 (12) | 0.40 ± 0.05 (40) | 0.94 ± 0.74 (12) | 0.58 ± 0.14 (17) | >100 (4) | >100 (4) | >100 (4) | >100 (5) | 0.06 ± 0.02 (11) |
| Max (%) | 104 ± 20.8 | 106 ± 1.47 | 67.8 ± 8.64 | 82.0 ± 7.49 | NA | NA | NA | NA | 68.2 ± 6.26 | |
| TrkC (rat) NIH3T3 | EC50 nM | 4.97 (2) | 0.21 ± 0.08 (7) | 0.65 ± 0.42 (6) | 0.67 ± 0.44 (5) | >100 (2) | >100 (2) | >100 (2) | >100 (2) | 0.22 ± 0.15 (7) |
| Max (%) | 76.2 | 103 ± 2.52 | 56.8 ± 8.16 | 82.3 ± 5.36 | NA | NA | NA | NA | 76.9 ± 7.68 | |
| NGF | NT-3 | BDNF | M2 | M3 | M4 | M5 | M6 | M7 | ||
| TrkA (human) NIH3T3 | EC50 nM | 0.97 ± 0.73 (4) | 4.35 ± 1.91 (4) | >100 (3) | ND | >100 (3) | ND | ND | ND | ND |
| Max (%) | 106 ± 5.65 | 73.6 ± 5.08 | NA | ND | NA | ND | ND | ND | ND |
(Top) M-antibodies have similar effects on human and rat Trk receptors as measured by phospho-ERK AlphaLISA. No statistically significant differences were detected when comparing the M-antibodies between the human and corresponding rat cell line. (Bottom) A phospho-ERK response was detected in cells expressing TrkA when treated with NGF or NT-3, but not BDNF or M3. ND: Not Done; NA: Not Applicable; Numbers in parentheses indicate the number of times the experiment was performed.