Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In-gel kinase assay of AtPR5K2. The recombinant fusion protein of the AtPR5K2 kinase domain (wPR5K2KD) or the mutagenized AtPR5K2 kinase domain (mPR5K2KD) was denatured and separated on an SDS gel. An in-gel kinase assay was performed using recombinant ABI1 (A; left), ABI1S314A (A; right), ABI2 (B; left), or ABI2S304A (B; right) as an embedded substrate. The GST tag served as a negative control.
