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. Author manuscript; available in PMC: 2020 Feb 13.
Published in final edited form as: Oncogene. 2019 Aug 13;38(44):7017–7034. doi: 10.1038/s41388-019-0923-2

Figure 6. LSD1 is a metastasis suppressor.

Figure 6.

(a) Kaplan-Meier curves showing reduced survival of MMTV-PyMT;Lsd1L/L;R26Y female mice with intraductal injection of Ad-K8-Cre at 6, 8, 12-weeks of age (n=7, 3, 4, respectively); when mice were injected at older ages (e.g., 12-weeks), they have already developed mammary tumors and the efficiency of infection by the injected adenovirus was probably reduced due to larger sizes of tumors. Control represents MMTV-PyMT;R26Y female mice (n=4) without intraductal injection of Ad-K8-Cre.

(b) Numbers of metastatic lesions found in the lungs of MMTV-PyMT;Lsd1L/L;R26Y mice (PyMT;Lsd1-null) and MMTV-PyMT;R26Y control mice (PyMT;Lsd1-wt) with intraductal injection of Ad-K8-Cre at ~4 weeks of age. Animals were analyzed ~2-3 months after injection. Data represent mean ± SEM, **: P<0.01.

(c) Left: Hematoxylin and eosin (H&E) pictures showing representative metastatic lesions (arrows) detected in lungs of mice with the indicated genotypes (as in b), scale bars = 50μm. Right: IF staining for LSD1 on lung metastasis from mice with the indicated genotypes, scale bars = 25μm.

(d) qRT-PCR analysis (normalized to Gapdh) of sorted YFP+ cells from PyMT primary tumors with or without LSD1-loss. Data represent mean ± SEM, n=3 or 4 independently sorted samples.