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. 2019 Oct 25;9:1034. doi: 10.3389/fonc.2019.01034

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Copyright © 2019 Hlavackova, Pilatova, Cerna, Selingerova, Mudry, Mazanek, Fedorova, Merhautova, Jureckova, Semerad, Pacasova, Flajsarova, Souckova, Demlova, Sterba, Valik and Zdrazilova-Dubska.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of two monocyte isolation modalities with respect to dendritic cell (DC) production. Elutriation (white box plots) and adherence to plastic (gray box plots) were compared based on QC parameters: (A) DC yield, and post-thaw: (B) viability, (C) DC phenotype on day 0: CD14, CD197, CD80, CD86, and CD83 and on day 2: MHC II, CD80, CD86, and CD83, and immunostimulatory properties presented by (D) IL-12 production, IL-10 production, and IL-12/IL-10 production ratio, (E) allo-MLR and auto-MLR. Median values are shown for each parameter for each monocyte isolation modality. Black dots show QC results of manufactured DCs that passed quality control, and red dots show results of manufactured DCs that did not pass quality control.