Figure 4.

Cluster analysis of DC parameters in the context of therapy prior to monocyte harvest. The heatmap on the right shows the immunostimulatory properties of manufactured DCs centered and scaled in the column direction (Z-score of parameters). Clusters are based on correlations. For clustering of DC parameters, but not batches, an equal meaning to positive and negative correlations was considered, and therefore strongly correlated parameters in the positive or negative manner clustered together. The left panel shows the treatment administered within 60 days of monocyte harvest. The day of the mononuclear harvest was set as day 0. An interactive version of the left panel with a detailed description of treatment including dosing is provided in Supplementary Material 1. Metronomic doses of chemotherapeutic drugs and supportive therapy such as vitamins and probiotics are not shown here but are summarized in Supplementary Table 2. Batches that did not pass quality control are indicated in red.